|
| Status |
Public on Sep 01, 2014 |
| Title |
4_3_2 |
| Sample type |
RNA |
| |
|
| Source name |
pre-implantatio embryo
|
| Organism |
Mus musculus |
| Characteristics |
cell type: 4-cell
strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell was lysed by freezing at -80 Celcius degree
|
| Label |
EvaGreen
|
| Label protocol |
The cells and pools were subjected to reverse transcription with SuperScript®VILO™ cDNA Synthesis kit (Life Technologies) as recommended by the manufacturer. DELTAgene™ Assays were custom designed for 94 genes associated with embryo development or regulation of gene expression, and two controls. The cDNA from cells and pools were subjected to specific target amplification for 20 cycles. Pre-amplified template was diluted and subjected to primer specific amplification with SooFast™EvaGreen®Supermix (Biorad) on 96.96 array chip and BiomarkHD™ System (Fluidigm).
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Data processing |
Raw Ct values = 999 were converted to 0 (zero). Relative quantification of gene expression (log2 space) was obtained by subtracting the Ct values from the baseline value of 22. Values < 0 (zero) were converted to 0 (zero).
|
| |
|
| Submission date |
Jul 29, 2014 |
| Last update date |
Sep 01, 2014 |
| Contact name |
Fernando H Biase |
| E-mail(s) |
fbiase@vt.edu
|
| Phone |
540-231-9520
|
| Organization name |
Virginia Tech
|
| Department |
School of Animal Sciences
|
| Lab |
Biase lab
|
| Street address |
175 West Campus Drive
|
| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24061 |
| Country |
USA |
| |
|
| Platform ID |
GPL19012 |
| Series (1) |
| GSE59892 |
Real-time quantitative PCR analysis of mouse pre-implantation embryos |
|
