|
| Status |
Public on Jul 31, 2014 |
| Title |
female-resting3 |
| Sample type |
RNA |
| |
|
| Source name |
male_resting macrophage cells
|
| Organism |
Gallus gallus |
| Characteristics |
developmental stage: newly-hatched; 1-3 days old
gender: female
cell type: Macrophages
cell status: resting
|
| Treatment protocol |
Cells were cultured in standard medium or with LPS to activate the macrophages.
|
| Growth protocol |
Fertilised chicken eggs were incubated at 38C with rocking.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNABee, following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Ambion WT labelling kit .
|
| |
|
| Hybridization protocol |
Hybridised on ChiGene 1.0 ST .
|
| Scan protocol |
GeneChip Scanner 3000 7G .
|
| Description |
gene expression in resting female
|
| Data processing |
Analysed with GeneChip® Command Console® Software (AGCC), applying RMA normalisation to the CEL files.
affymetrix-algorithm-name = rma
affymetrix-algorithm-version = 1.0
affymetrix-array-type = Chicken
program-name = Expression Console
program-version = 1.2.1.20
|
| |
|
| Submission date |
Jul 30, 2014 |
| Last update date |
Jul 31, 2014 |
| Contact name |
Sunil Nandi |
| E-mail(s) |
sunil.nandi@roslin.ed.ac.uk
|
| Phone |
01316519173
|
| Organization name |
The Roslin Institute
|
| Department |
Developmental Biology
|
| Lab |
Clinton
|
| Street address |
University of Edinburgh
|
| City |
Easter Bush |
| State/province |
Midlothian |
| ZIP/Postal code |
EH25 9RG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL3213 |
| Series (2) |
| GSE59920 |
Macrophages from newly hatched chicks [set2] |
| GSE59921 |
Macrophages from male and female chickens |
|
