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Sample GSM146185 Query DataSets for GSM146185
Status Public on Dec 25, 2006
Title TC.CD4-5
Sample type RNA
 
Source name Human subject, Control
Organism Homo sapiens
Characteristics Lymph-node biopsy
Treatment protocol Frozen samples homogenized on ice in the Trizol solution (GibcoBRL).
Growth protocol Frozen samples
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA and hybridized on the HG U133 2.0 plus GeneChip.
 
Hybridization protocol Following fragmentation, 15 mg of fragmented cRNA were hybridized to HG-U133 2.0 plus microarrays (Affymetrix, Inc.). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000.
Description Gene expression data from PTCL/U patient at diagnosis
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Nov 17, 2006
Last update date Aug 28, 2018
Contact name pier paolo piccaluga
E-mail(s) ppicca@med.unibo.it
Organization name university of bologna
Street address via massarenti 9
City bologna
ZIP/Postal code 40138
Country Italy
 
Platform ID GPL570
Series (1)
GSE6338 Gene expression analysis of Peripheral T-cell Lymphoma/Unspecified
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE65823
Reanalyzed by GSE86362
Reanalyzed by GSE119087

Data table header descriptions
ID_REF Probeset ID of Affymetrix chip
VALUE 'signal' a meature of the abundance of a transcript (obtained using GCOS1.2); processed as GC-RMA and norrmalized per gene to median and per chip to 50th percentile in GeneSpring 3.7.1 (Agilent Tecnology)
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1007_s_at 0.9688021 A
1053_at 1.72485 P
117_at 0.81161505 A
121_at 0.73238987 P
1255_g_at 0.79765916 A
1294_at 0.7014509 P
1316_at 1.3353848 P
1320_at 0.79765916 A
1405_i_at 0.120250106 P
1431_at 0.74371654 A
1438_at 0.7841893 A
1487_at 1.1740578 A
1494_f_at 1.9473684 P
1552256_a_at 1.5107871 P
1552257_a_at 0.8778118 P
1552258_at 1.2481673 P
1552261_at 1.2338787 A
1552263_at 0.68191284 P
1552264_a_at 0.41679537 A
1552266_at 2.3836257 A

Total number of rows: 54675

Table truncated, full table size 1229 Kbytes.




Supplementary file Size Download File type/resource
GSM146185.CEL.gz 7.9 Mb (ftp)(http) CEL

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