|
| Status |
Public on Nov 21, 2014 |
| Title |
Cortex tissue from WT mice harvested at ZT4 replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Cortex tissue ZT4
|
| Organism |
Mus musculus |
| Characteristics |
tissue: cortex
time: ZT4
strain: C57/bl6
genotype: wildtype
age: 4 months
|
| Treatment protocol |
Mice were anesthetized, immediatley decapitated and cortical tissue (bregma 0 to -2mm) was isolated using a ‘rodent brain matrix’ 1 mm coronal slicer.
|
| Growth protocol |
Mice were group housed under 12h light 12 dark conditions in standard cages and harvested at indicated timepoints.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U74v2 Mouse Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station according to manufacturer protocols.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Expression data from adult mouse cortex
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jul 30, 2014 |
| Last update date |
Nov 21, 2014 |
| Contact name |
Moritz J Rossner |
| E-mail(s) |
moritz.rossner@med.uni-muenchen.de
|
| Organization name |
Lud.-Max.-University
|
| Department |
Psychiatry
|
| Lab |
Molecular Neurobiology
|
| Street address |
Nussbaumstr. 7
|
| City |
Munich |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE59941 |
Expression data from adult mouse cortex harvested at two different zeitgeber (ZT) timepoints of the 24h cycle |
|
