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Status
Public on Mar 21, 2004
Title
267B1 vs. PC3M. Array 2.
Sample type
genomic
Channel 1 Source name
prostate cell line PC3M DNA, Cy5
Organism
Homo sapiens Extracted molecule
genomic DNA
Channel 2 Source name
prostate cell line 267B1 DNA, Cy3
Organism
Homo sapiens Extracted molecule
genomic DNA
Description
The methylation status of 267B1 and PC3M are compared. DNA samples were digested with methylation sensitive enzyme HpaII, ligated with adaptor, PCR amplified and labeled with Cye Dye. The labeled target are hybridized with human promoter array. In general, methylated sequences will not be cut and will not be amplified. Only unmethylated sequences will be amplified and hybridized to arrays.
Submission date
Dec 30, 2003
Last update date
May 28, 2005
Contact name
Yipeng Wang
E-mail(s)
ywang@sdibr.org Phone
8585813960
Organization name
Vaccine Research Institute of San Diego
Street address
City
San Diego
State/province
CA
ZIP/Postal code
92121
Country
USA
Platform ID
GPL864 Series (1)
GSE951 267B1, PC3M, LNCaP Methylation Profile vs. mRNA Expression Level
Data table header descriptions ID_REF
CH1_RAW channel 1 intensity
CH1_BKD_RAW ch1 background
CH2_RAW channel 2 intensity
CH2_BKD_RAW ch2 background
VALUE calculation
Data table ID_REF CH1_RAW CH1_BKD_RAW CH2_RAW CH2_BKD_RAW VALUE 1
213.479996
0
436.51001
8.8
-0.346979672
2
160.669998
0.933333
526.309998
14.666667
-1.019837764
3
280.299988
0
582.580017
7.033333
-0.415399173
4
6330.370117
0.2
7952.919922
2.9
-0.202676897
5
572.219971
0
985.909973
0
-0.203270252
6
270.630005
0
881.200012
39.966667
-1.008865216
7
570.090027
0
484.540009
0
0.867772596
8
313.5
0
357.76001
0
0.456298921
9
308.079987
8.5
560.150024
0
-0.282537715
10
257.299988
0.9
517.97998
0
-0.378075295
11
237.089996
0
372.540009
0
0.004809035
12
1473.199951
0
1234.670044
0
0.603294912
13
1059.640015
0
836.640015
0
0.848083525
14
655.429993
6.4
1060.839966
0
-0.158139189
15
781.02002
0
1059.160034
0
0.080958783
16
349.070007
0
594.169983
0
-0.143477732
17
440.790009
0
533.549988
2.233333
0.358732767
18
664.950012
0
864.340027
0
0.200727359
19
245.589996
0
343.779999
0
0.173178577
20
327.109985
0
559.200012
0
-0.154925911
17328 807 Kbytes .Supplementary data files not provided
