|
| Status |
Public on Aug 02, 2014 |
| Title |
Group C_Experiment_30 days_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
primary lung adenocarcinoma
|
| Organism |
Mus musculus |
| Characteristics |
strain: Lox-stop-lox Kras G12D mice
infected with: adenovirus CRE (Ad-Cre)
tissue: lung
tissue type: primary lung adenocarcinoma
cell type: Sca-1+CD34+ cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA are harvested using TRIzol (Invitrogen) and RNeasy mini kit (QIAGEN) according to manufacturer’s instructions.
|
| Label |
Hy3
|
| Label protocol |
After having passed RNA measurement on the Nanodrop instrument, the samples are labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit and hybridized on the miRCURY™ LNA Array (v.11.0).
|
| |
|
| Hybridization protocol |
The samples were hybridized on a hybridization station following the scheme you outlined in the sample submission.
|
| Scan protocol |
Scanning is performed with the Axon GenePix 4000B microarray scanner. GenePix pro V6.0 is used to read the raw intensity of the image.
|
| Data processing |
The intensity of green signal is calculated after background subtraction and replicated spots on the same slide have been averaged by getting a median intensity. We use Median Normalization Method to obtain “Normalized Data”, Normalized Data=(Foreground-Background)/median, the median is 50 percent quantile of microRNA intensity which is larger than 50 in all samples after background correction.
|
| |
|
| Submission date |
Aug 01, 2014 |
| Last update date |
Aug 02, 2014 |
| Contact name |
guo zhen zhao |
| E-mail(s) |
zlpjyo@126.com
|
| Organization name |
Cancer Institute of PLA, Xinqiao Hospital, Third Military Medical University,
|
| Street address |
Cancer Institute of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing, China
|
| City |
chongqing |
| ZIP/Postal code |
400037 |
| Country |
China |
| |
|
| Platform ID |
GPL19029 |
| Series (1) |
| GSE60001 |
MicroRNA profile of tumorigenic cells during formation of pulmonary adenocarcinoma |
|
