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Sample GSM1463413 Query DataSets for GSM1463413
Status Public on Aug 02, 2014
Title Group C_Experiment_30 days_rep4
Sample type RNA
Source name primary lung adenocarcinoma
Organism Mus musculus
Characteristics strain: Lox-stop-lox Kras G12D mice
infected with: adenovirus CRE (Ad-Cre)
tissue: lung
tissue type: primary lung adenocarcinoma
cell type: Sca-1+CD34+ cells
Extracted molecule total RNA
Extraction protocol Total RNA are harvested using TRIzol (Invitrogen) and RNeasy mini kit (QIAGEN) according to manufacturer’s instructions.
Label Hy3
Label protocol After having passed RNA measurement on the Nanodrop instrument, the samples are labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit and hybridized on the miRCURY™ LNA Array (v.11.0).
Hybridization protocol The samples were hybridized on a hybridization station following the scheme you outlined in the sample submission.
Scan protocol Scanning is performed with the Axon GenePix 4000B microarray scanner. GenePix pro V6.0 is used to read the raw intensity of the image.
Data processing The intensity of green signal is calculated after background subtraction and replicated spots on the same slide have been averaged by getting a median intensity. We use Median Normalization Method to obtain “Normalized Data”, Normalized Data=(Foreground-Background)/median, the median is 50 percent quantile of microRNA intensity which is larger than 50 in all samples after background correction.
Submission date Aug 01, 2014
Last update date Aug 02, 2014
Contact name guo zhen zhao
Organization name Cancer Institute of PLA, Xinqiao Hospital, Third Military Medical University,
Street address Cancer Institute of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing, China
City chongqing
ZIP/Postal code 400037
Country China
Platform ID GPL19029
Series (1)
GSE60001 MicroRNA profile of tumorigenic cells during formation of pulmonary adenocarcinoma

Data table header descriptions
VALUE Normalized signal intensity

Data table
46761 0.078465562
46774 0.706190061
46615 0.001743679
46289 0.02092415
46773 -0.003487358
46764 0.006974717
46816 0.029642546
46279 -0.001743679
46844 0.005231037
46217 0.029642546
46331 0.003487358
46572 -0.001743679
46724 0
46658 0.001743679
46293 0.001743679
46683 0.003487358
46610 0
46636 0
46283 -0.001743679
46457 0

Total number of rows: 650

Table truncated, full table size 11 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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