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GEO accession:
Sample GSM146362
Query DataSets for GSM146362
Status
Public on Mar 31, 2007
Title
Golden Promise Root Salt, biological rep 3
Sample type
RNA
Source name
Golden Promise Root
Organism
Hordeum vulgare
Characteristics
Genotype: Golden Promise
Treatment protocol
salt root
Growth protocol
hydroponics
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol
GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner
Description
Gene expression data from barley root
Data processing
The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method.
Submission date
Nov 20, 2006
Last update date
Mar 31, 2007
Contact name
Harkamal Walia
Organization name
University of Nebraska
Street address
Agronomy and Horticulture
City
Lincoln
State/province
NE
ZIP/Postal code
68583
Country
USA
Platform ID
GPL1340
Series (1)
GSE6325
Array-based genotyping and expression analysis of barley cv. Maythorpe and Golden Promise
Data table header descriptions
ID_REF
VALUE
GCOS-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
150.3
P
0.00762
AFFX-BioB-M_at
92.1
P
0.001102
AFFX-BioB-3_at
102
P
0.001102
AFFX-BioC-5_at
413.6
P
0.00006
AFFX-BioC-3_at
430.9
P
0.000044
AFFX-BioDn-5_at
735.2
P
0.000044
AFFX-BioDn-3_at
2109.5
P
0.000147
AFFX-CreX-5_at
3950.2
P
0.000044
AFFX-CreX-3_at
6863.1
P
0.000044
AFFX-DapX-5_at
257.7
P
0.00007
AFFX-DapX-M_at
638.7
P
0.000195
AFFX-DapX-3_at
1444.2
P
0.000044
AFFX-LysX-5_at
59.7
P
0.000972
AFFX-LysX-M_at
118.3
P
0.030976
AFFX-LysX-3_at
283.1
P
0.000044
AFFX-PheX-5_at
91.6
P
0.000297
AFFX-PheX-M_at
94.2
P
0.002275
AFFX-PheX-3_at
152.8
P
0.000258
AFFX-ThrX-5_at
86.7
P
0.002556
AFFX-ThrX-M_at
155.9
P
0.000095
Total number of rows:
22840
Table truncated, full table size
703 Kbytes
.
Supplementary file
Size
Download
File type/resource
GSM146362.CEL.gz
3.5 Mb
(ftp)
(http)
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