|
| Status |
Public on Aug 05, 2014 |
| Title |
rxLeptin injection juvenile frog 5 |
| Sample type |
RNA |
| |
|
| Source name |
Juvenile X. laevis frogs
|
| Organism |
Xenopus laevis |
| Characteristics |
Stage: juvenile
tissue: preoptic area/hypothalamus/pituitary
avg body weight: 0.8 g
treatment: rxLeptin injection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction with Trizol followed by Qiagen total RNA extraction columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the Ambion Premier kit protocol from 250 ng total RNA (MessageAmp Premier RNA Amplification Kit Instruction Manual, 2008, Ambion)
|
| |
|
| Hybridization protocol |
Following fragmentation, 16 ug of aRNA were hybridized for 16 hr at 45C on Xenopus Genome 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
|
| Description |
Gene expression data from frogs given i.c.v. rxLeptin injection
|
| Data processing |
Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
|
| |
|
| Submission date |
Aug 04, 2014 |
| Last update date |
Aug 05, 2014 |
| Contact name |
Robert J Denver |
| E-mail(s) |
rdenver@umich.edu
|
| Phone |
7349366625
|
| Organization name |
University of Michigan
|
| Department |
MCDB
|
| Street address |
1105 North University Ave
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109-1085 |
| Country |
USA |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE60053 |
Acute (2 hr) response to rxLeptin injection in preoptic area/hypothalamus/pituitary of juvenile Xenopus laevis |
|
