|
| Status |
Public on Apr 15, 2015 |
| Title |
orthoxenograft passage 4, from NF1-associated MPNST number 1 |
| Sample type |
RNA |
| |
|
| Source name |
MPNST orthoxenograft
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: orthoxenograft
host organism: nude mouse
nf1-associated: Yes
original tumor: NF_001
passage: 4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen samples using miRCURY RNA (Exiqon). RNA integrity number (RIN) was verified for each sample using a RNA Nano Chip Kit (Agilent Technologies, Germany) in Agilent Bioanalyzer 2100.
|
| Label |
biotin
|
| Label protocol |
The Ambion WT Expression Kit and the Affymetrix GeneChip WT terminal labeling
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized to Human GEneCHip Gene 1.0 ST microarray (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
The array were scanned using the Affymetrix Model 3000 Scanner.
|
| Description |
orthoxenograft passage 4, from NF1-associated MPNST number 1
|
| Data processing |
The data were processed using R/Bioconductor using the packages “gcrma” and “hugene10stv1cdf”. The rma function was used to normalize the data.
|
| |
|
| Submission date |
Aug 04, 2014 |
| Last update date |
Apr 16, 2015 |
| Contact name |
Bernat Gel Moreno |
| E-mail(s) |
bgel@imppc.org
|
| Phone |
+34935543068
|
| Organization name |
Institut de Medicina Predictiva i Personalitzada del Càncer
|
| Department |
Hereditary Cancer Program
|
| Lab |
Genetic Variation and Cancer
|
| Street address |
Ctra. de Can Ruti, camí de les escoles, s/n
|
| City |
Badalona |
| State/province |
Barcelona |
| ZIP/Postal code |
08916 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE60082 |
Effective sorafenib treatment response in a panel of genomically-characterized malignant peripheral nerve sheath tumor orthoxenograft models |
|
