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Status |
Public on Nov 30, 2016 |
Title |
FAPs_5'ss_AMO_PDGF_50_rep2 |
Sample type |
RNA |
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Source name |
Fibro/adipogenic progenitors isolated from hindlimb skeletal muscles, treated with 5'ss AMO and 50uM PDGF-AA
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Organism |
Mus musculus |
Characteristics |
gender: male tissue: skeletal muscle cell type: fibro/adipogenic progenitors age: 8-12 weeks strain: C57/Bl6 treatment: 5' Splice Site AMO, 50uM PDGF-AA
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Treatment protocol |
The media was switched to Ham’s F10 supplemented with 10% horse serum and transfected with 10 µM AMO as indicated for 48 hours. The media was then replaced with Opti-Mem and cells re-transfected with 10 µM AMO with 50uM PDGF-AA or control. After 48 hours, total RNA was extracted.
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Growth protocol |
Cells were grown for 2.5 days in DMEM supplemented with 10% FBS prior to treatment.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit as per the manufacturer’s instructions (Qiagen).
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Label |
biotin
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Label protocol |
Biotinylated single stranded cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
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Hybridization protocol |
Fragmented biotin-labeled single stranded DNA was hybridized to Affymetrix Mouse Gene 1.0 Array according to the standard Affymetrix protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
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Description |
Gene expression data from WT fibro/adipogenic progenitors isolated from a noninjured animal and treated in vitro with Intronic PDGFRα 5' splice site AMO and 50uM PDGF-AA.
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Data processing |
The data were analyzed with the Affymertix Expression Console software using Affymetrix default RMA analysis settings.
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Submission date |
Aug 05, 2014 |
Last update date |
Nov 30, 2016 |
Contact name |
Thomas A Rando |
E-mail(s) |
rando@stanford.edu
|
Organization name |
Stanford University School of Medicine
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Department |
Neurology and Neurological Sciences
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Street address |
3801 Miranda Ave
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City |
Palo Alto |
State/province |
CA |
ZIP/Postal code |
94304 |
Country |
USA |
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Platform ID |
GPL6246 |
Series (1) |
GSE60099 |
Intronic polyadenylation of PDGFRα in resident stem cells attenuates muscle fibrosis |
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