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Sample GSM146614 Query DataSets for GSM146614
Status Public on Nov 06, 2007
Title Multinodular goiter, sample 33
Sample type RNA
 
Source name Homo sapiens thyroid, Multinodular goiter
Organism Homo sapiens
Characteristics Gender: male
Age: 39 years
Thyroïditis: no
Metastasis: no
Nucleus type: Follicular
Nodule type: Multiple
Vesicle type: Macrofollicular
Connective tissue type: Normal
Filter ID: 03
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from tissue samples using a standard guanidium isothiocyanate protocol (TRIzol reagent, Life Technologies, Inc., Gaithersburg, MD, USA)
Label [alpha-33P]deoxy-CTP
Label protocol the array was hybridized with a complex target prepared from 5 ?g total RNA by simultaneous RT and [alpha-33P]deoxy-CTP labelling as described by the manufacturer.
 
Hybridization protocol the array was hybridized with a complex target prepared from 5 ?g total RNA by simultaneous RT and [alpha-33P]deoxy-CTP labelling as described by the manufacturer.
Scan protocol hybridization images were obtained by scanning with an imaging plate device (Fuji BAS 5000, Raytest, Paris, France). Signal intensities were quantified using ArrayGauge software (Fujifilm Medical Systems, Stanford, CT, USA).
Description gene expression data from thyroid tissue diagnosed as Multinodular goiter
Data processing data were subjected to print-tip Lowess normalization and median normalization
 
Submission date Nov 21, 2006
Last update date Nov 05, 2007
Contact name Jean-Fred Fontaine
E-mail(s) jean-fred.fontaine@univ-angers.fr
Phone +33 (0)2 41 35 33 14
Fax +33 (0)2 41 35 40 17
Organization name INSERM
Lab Biochimie
Street address 4 rue Larrey
City angers
ZIP/Postal code 49033
Country France
 
Platform ID GPL4512
Series (1)
GSE6339 Expression data from 12 human thyroid tissue classes and 2 cell lines

Data table header descriptions
ID_REF
VALUE normalized signal intensities

Data table
ID_REF VALUE
1
2
3
4
5
6
7 0.795961884
8
9 0.599957133
10 1.157969438
11 0.734531626
12 0.822357302
13 0.71249958
14
15 1.015250993
16
17
18
19
20

Total number of rows: 9216

Table truncated, full table size 116 Kbytes.




Supplementary file Size Download File type/resource
GSM146614_probes.txt.gz 59.6 Kb (ftp)(http) TXT
GSM146614_sample.txt.gz 58.4 Kb (ftp)(http) TXT

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