|
| Status |
Public on Dec 01, 2006 |
| Title |
Actinomycin D treated A549 human lung cancer cell cultures replicate A |
| Sample type |
RNA |
| |
|
| Source name |
Actinomycin D treated A549 human lung cancer cell cultures replicate A
|
| Organism |
Homo sapiens |
| Characteristics |
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Growth protocol |
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
|
| Label |
Affymetrix single-stage biotin
|
| Label protocol |
We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
|
| |
|
| Hybridization protocol |
We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
|
| Scan protocol |
We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
|
| Description |
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Data processing |
RMA Normalization
|
| |
|
| Submission date |
Nov 25, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Joseph Gerard Hacia |
| E-mail(s) |
hacia@hsc.usc.edu
|
| Phone |
323-442-3030
|
| Organization name |
University of Southern California
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Hacia Lab
|
| Street address |
2250 Alcazar Street, IGM 261
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE6400 |
Cultured A549 lung cancer cells treated with actinomycin D and sapphyrin PCI-2050 |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
