|
| Status |
Public on Aug 03, 2015 |
| Title |
13732473_2007 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
primary cortical neurons
|
| Organism |
Mus musculus |
| Characteristics |
treatment: NLM (nutrient limited media)
cell type: primary cortical neurons
strain: C57BL/6J
|
| Biomaterial provider |
University of Washington (Seattle, WA, USA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy kit (Qiagen) following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
RNA was 3' end labeled with Hy3 following the Exiqon miRCURY LNA Labeling Kit protocol.
|
| |
|
| Channel 2 |
| Source name |
primary cortical neurons
|
| Organism |
Mus musculus |
| Characteristics |
treatment: CM (complete media)
cell type: primary cortical neurons
strain: C57BL/6J
|
| Biomaterial provider |
University of Washington (Seattle, WA, USA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy kit (Qiagen) following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
RNA was 3' end labeled with Hy5 following the Exiqon miRCURY LNA Labeling Kit protocol.
|
| |
|
| |
| Hybridization protocol |
Hybridizations were performed using Exiqon's protocol.
|
| Scan protocol |
Arrays were scanned using the Axon 4000B Scanner through the Functional Genomics Laboratory (UW).
|
| Description |
Using sets of wild-type C57BL/6J mice, we established primary cortical neuron cultures from P0 littermates, and cultured these neurons (n = 3 / set) in CM or NLM for 4 hrs.
|
| Data processing |
Genepix (version Pro 6.0.1.00)
|
| |
|
| Submission date |
Aug 15, 2014 |
| Last update date |
Sep 05, 2017 |
| Contact name |
James William MacDonald |
| E-mail(s) |
jmacdon@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL7724 |
| Series (1) |
| GSE60452 |
Let-7 coordinately suppresses components of the amino acid sensing pathway to induce autophagy |
|
