|
| Status |
Public on Nov 01, 2008 |
| Title |
Cell line HepG2(b) |
| Sample type |
RNA |
| |
|
| Source name |
cell line
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
|
| Description |
Gene expression data for cell line
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Dec 06, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Kun Yu |
| E-mail(s) |
yukun@omniarray.com
|
| Phone |
+65 64368390
|
| Organization name |
National Cancer Centre
|
| Department |
Cellular & Molecular Research
|
| Street address |
|
| City |
Singapore |
| ZIP/Postal code |
169610 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE6465 |
Expression data of Hepatocellular Carcinoma |
|
| Relations |
| Reanalyzed by |
GSE51798 |
| Reanalyzed by |
GSE119087 |
