|
| Status |
Public on Jun 30, 2015 |
| Title |
hMSC cell line expressing EWSR1-NFATc2 (replicate2) |
| Sample type |
RNA |
| |
|
| Source name |
hMSC cell line expressing EWSR1-NFATc2 (replicate2)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: hMSC cell line
growth medium: RPMI-1640 medium supplemented with 10% foetal calf serum
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol using 2µg total RNA
|
| |
|
| Hybridization protocol |
As according to the manufacturer's instructions.
|
| Scan protocol |
GCOS 1.4
|
| Description |
Gene expression data of hMSC cell line expressing EWSR1-NFATc2 (replicate2)
|
| Data processing |
All data were normalized using the gcrma package version 2.34.0 in the R 3.0.2 environment (R Development Core Team, 2005) with Brainarray Entrez gene CDF v17 and with quantile normalisation
|
| |
|
| Submission date |
Aug 25, 2014 |
| Last update date |
Jun 30, 2015 |
| Contact name |
Franck Tirode |
| Organization name |
Institut Curie
|
| Department |
Inserm U830
|
| Lab |
Génétique et biologie des tumeurs pédiatriques
|
| Street address |
26 Rue d'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL17811 |
| Series (1) |
| GSE60740 |
Expression profiles of Ewing family of tumors authenticate distinct molecular entities |
|
