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Sample GSM1494831 Query DataSets for GSM1494831
Status Public on Nov 06, 2015
Title PDAC_13 [miRNA]
Sample type RNA
 
Source name Fresh frozen tumor
Organism Homo sapiens
Characteristics tissue: pancreatic ductal adenocarcinoma
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cell pellets using mirVana kit (Ambion/Life Technologies) according to the manufacturer’s instructions. Total RNA was eluted in 100 µl H2O, concentration measured on NanoDrop ND-1000.
Label Cy3
Label protocol miRNA was labeled with Cy-3 according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies, using 200 ng total RNA as input.
 
Hybridization protocol miRNA microarrays Cy-3-labeled cRNA was hybridized according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies.
Scan protocol The slides were scanned immediately after washing on the Agilent Microarray Scanner G2565C using protocol GE1_107_Sep09 for the expression microarrays and protocol.
Description Gene expression of fresh frozen pancreatic ductal adenocarcinoma patient 13
Data processing The scanned images were analyzed using Feature Extraction Software 10.7.3.1 (Agilent Technologies) using default parameters. The expression profiles was RMA normalized for miRNA expression data. Filtering of controls, unannotated genes and low expressed probes was done.
 
Submission date Sep 02, 2014
Last update date Nov 06, 2015
Contact name Vandana Sandhu
E-mail(s) vansan@rr-research.no
Organization name Radiumhospitalet, Oslo
Department Department of Genetics
Street address Ullernchausseen 70
City Oslo
State/province oslo
ZIP/Postal code 0310
Country Norway
 
Platform ID GPL15159
Series (2)
GSE60978 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. [miRNA]
GSE60980 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_25_P00015345 4.601481396
A_25_P00015343 5.097096838
Blank 4.26340652
dmr_285 12.01014063
dmr_3 14.28963873
dmr_308 4.689506902
dmr_316 4.686548172
dmr_31a 11.55947674
dmr_6 13.58600911
A_25_P00013722 4.658479157
A_25_P00013718 4.727261363
A_25_P00011668 4.850014972
A_25_P00013809 4.647085074
A_25_P00011834 4.627064086
A_25_P00013749 4.65426099
A_25_P00013752 5.977429867
A_25_P00011853 6.651706447
A_25_P00013814 4.542918985
A_25_P00014783 4.649464138
A_25_P00011886 4.606014884

Total number of rows: 1368

Table truncated, full table size 35 Kbytes.




Supplementary file Size Download File type/resource
GSM1494831_miRNA_PDAC_13.txt.gz 8.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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