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Sample GSM1494886 Query DataSets for GSM1494886
Status Public on Nov 06, 2015
Title ampullaP_2 [miRNA]
Sample type RNA
 
Source name Fresh frozen tumor
Organism Homo sapiens
Characteristics tissue: ampulla pancreatobiliary type
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cell pellets using mirVana kit (Ambion/Life Technologies) according to the manufacturer’s instructions. Total RNA was eluted in 100 µl H2O, concentration measured on NanoDrop ND-1000.
Label Cy3
Label protocol miRNA was labeled with Cy-3 according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies, using 200 ng total RNA as input.
 
Hybridization protocol miRNA microarrays Cy-3-labeled cRNA was hybridized according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies.
Scan protocol The slides were scanned immediately after washing on the Agilent Microarray Scanner G2565C using protocol GE1_107_Sep09 for the expression microarrays and protocol.
Description Gene expression of fresh frozen ampulla pancreatobiliary type patient 2
Data processing The scanned images were analyzed using Feature Extraction Software 10.7.3.1 (Agilent Technologies) using default parameters. The expression profiles was RMA normalized for miRNA expression data. Filtering of controls, unannotated genes and low expressed probes was done.
 
Submission date Sep 02, 2014
Last update date Nov 06, 2015
Contact name Vandana Sandhu
E-mail(s) vansan@rr-research.no
Organization name Radiumhospitalet, Oslo
Department Department of Genetics
Street address Ullernchausseen 70
City Oslo
State/province oslo
ZIP/Postal code 0310
Country Norway
 
Platform ID GPL15159
Series (2)
GSE60978 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. [miRNA]
GSE60980 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_25_P00015345 4.615346146
A_25_P00015343 4.679844244
Blank 4.311197116
dmr_285 11.45446072
dmr_3 12.44971512
dmr_308 4.691044702
dmr_316 4.714321704
dmr_31a 11.09931497
dmr_6 11.59675849
A_25_P00013722 4.654101906
A_25_P00013718 4.712328074
A_25_P00011668 4.803788951
A_25_P00013809 4.670571272
A_25_P00011834 4.595810672
A_25_P00013749 4.660503345
A_25_P00013752 5.714394652
A_25_P00011853 6.665405753
A_25_P00013814 4.558062931
A_25_P00014783 4.607104711
A_25_P00011886 4.543217531

Total number of rows: 1368

Table truncated, full table size 35 Kbytes.




Supplementary file Size Download File type/resource
GSM1494886_miRNA_ampullaP_2.txt.gz 8.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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