|
| Status |
Public on Mar 01, 2015 |
| Title |
CBF1OX low temperature A |
| Sample type |
RNA |
| |
|
| Source name |
CBF1OX low temperature
|
| Organism |
Solanum tuberosum |
| Characteristics |
cultivar: cv. Désirée
genotype/variation: AtCBF1OX transgenic
stress condition: low temperature
tissue: young leaves
|
| Treatment protocol |
Low temperature: To analyze the effect of low temperature conditions, plants were first grown under long days (16 h light/8 h darkness) with fluorescent illumination (50 µmol m-2 s-1) at 22 ºC. After one month, half of the plants were exposed to 4 ºC under similar light conditions, while the remaining plants were kept at 22 ºC.
High light: The effect of high light stress was evaluated on plants grown first under long day conditions in a growth chamber illuminated with high pressure sodium lamps at 50 µmol m-2 s-1, at 22 ºC. After one month, half of the plants were exposed to 800 µmol m-2 s-1 at 22 ºC, while the remaining plants were kept under 50 µmol m-2 s-1 at 22 ºC.
Drought: for drought stress, plants were grown under natural radiation in a greenhouse in 3 liter pots supplemented with daily irrigation until water soil capacity was saturated. After one month under this condition, irrigation was interrupted for half of the plants while the remaining plants continued with the previous daily irrigation regime.
|
| Growth protocol |
Potato plants (Solanum tuberosum cv. Désirée) were grown under sterile conditions in glass bottles containing Murashige and Skoog (MS, Sigma) medium solidified with 0.8% phytoagar (Sigma). Plants were propagated in vitro and grown under 16 h light (50 µmol m-2 s-1cool-white fluorescent illumination)/8 h dark cycles, at 22C. The analysis of physiological and molecular responses of plants to different stress treatments was conducted on plants transferred to plastic pots containing a mixture of perlite:vermiculite:peat moss (1:1:1), and acclimated for one month under the same light and temperature conditions in which the treatments were applied.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using RNaeasy Mini kit system according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Analysis Technical Manual, 2005, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Arabidopsis Genome ATH1 Array were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G
|
| Description |
CBF1LTA
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Sep 02, 2014 |
| Last update date |
Mar 01, 2015 |
| Contact name |
Marcelo Yanovsky |
| E-mail(s) |
myanovsky@leloir.org.ar
|
| Organization name |
Fundación Instituto Leloir. IIBBA- CONICET
|
| Lab |
Laboratorio de Genómica Comparativa del Desarrollo Vegetal
|
| Street address |
Av. Patricias Argentinas 435
|
| City |
Buenos Aires |
| State/province |
Ciudad Autonoma de Buenos Aires |
| ZIP/Postal code |
C1405BWE |
| Country |
Argentina |
| |
|
| Platform ID |
GPL4741 |
| Series (1) |
| GSE61004 |
Expression data from Wild type (WT) and AtCBF1 OX potato plants exposed to different abiotic stresses |
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