|
| Status |
Public on Dec 23, 2014 |
| Title |
Long-Evans_Hypothalamus_TCDD_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Long-Evans_Hypothalamus_TCDD
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Long-Evans
gender: male
age: 16-22 weeks
tcdd dose: 100 ug/kg
vehicle: corn oil
exposure length: 23 hours
|
| Treatment protocol |
Animals were treated with either 100 ug/kg TCDD in corn oil vehicle or corn oil alone by oral gavage.
|
| Growth protocol |
Animals were grown in a 12h/12h light/dark cycle, housed singly in suspended stainless-steel wire-mesh cages with pelleted R36 feed and tap water. The housing environment was maintained at 21 +/- 1 degree Celcius with relative humidity 50% +/- 10%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy Lipid Tissue Mini kits according to the manufacturer's instructions. Total RNA was quantified by UV spectrophotometry and RNA integrity was verified using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada.
|
| |
|
| Hybridization protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada.
|
| Scan protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada.
|
| Description |
LET4
|
| Data processing |
Quantitated array data (CEL files) were loaded into the R statistical environment (v3.1.0) using the affy package (v1.42.2) of the BioConductor library. Data was pre-processed with the RMA algorithm (Irizarry et al. NAR) and probes were mapped to Entrez Gene Ids using the rat2302rnentrezgcdf (v18.0.0) package in R.
|
| |
|
| Submission date |
Sep 03, 2014 |
| Last update date |
Dec 23, 2014 |
| Contact name |
Stephenie Prokopec |
| E-mail(s) |
stephenie.prokopec@oicr.on.ca
|
| Organization name |
Ontario Institute for Cancer Research
|
| Street address |
661 University Avenue, Suite 510
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL19144 |
| Series (1) |
| GSE61039 |
Transcriptional Profiling of rat hypothalamic response to TCDD |
|
