|
| Status |
Public on Jun 02, 2015 |
| Title |
Dog-HNSCC-1172 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
DNA from HNSCC tumor tissue of dog 1172
|
| Organism |
Canis lupus familiaris |
| Characteristics |
genotype: Head and neck squamous cell carcinoma
cell type: HNSCC
strain: Unknown
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from the dissected tissues using a Qiagen Tissue DNeasy purification kit (exactly following the kit manual)
|
| Label |
Cy3
|
| Label protocol |
Labeling test samples with Cy3 following the NimbleGen official Manual, see more at NimbleGen website, and labeling reference samples with Cy5 following the NimbleGen official Manual, see more at NimbleGen website.
|
| |
|
| Channel 2 |
| Source name |
DNA from head and neck normal tissue of dog 1172
|
| Organism |
Canis lupus familiaris |
| Characteristics |
genotype: Normal
cell type: Normal cell
strain: Unknown
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from the dissected tissues using a Qiagen Tissue DNeasy purification kit (exactly following the kit manual)
|
| Label |
Cy5
|
| Label protocol |
Labeling test samples with Cy3 following the NimbleGen official Manual, see more at NimbleGen website, and labeling reference samples with Cy5 following the NimbleGen official Manual, see more at NimbleGen website.
|
| |
|
| |
| Hybridization protocol |
Using NimbleGen Hybridization System (following the NimbleGen official Manual)
|
| Scan protocol |
Scanning two-color NimbleGen arrays with GenePix 4000B Scanner and associated software(following the NimbleGen official Manual)
|
| Data processing |
Analyzing the data with the segMNT algorithm (following the NimbleGen official Manual), and background correction as implemented in the NimbleScan software package, version 2.6.0.0 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Sep 08, 2014 |
| Last update date |
Jun 03, 2015 |
| Contact name |
Deli Liu |
| E-mail(s) |
del2017@med.cornell.edu
|
| Organization name |
Weill Cornell Medicine
|
| Street address |
1300 York Ave
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL9314 |
| Series (1) |
| GSE61231 |
Canine Spontaneous Head and Neck Squamous Cell Carcinomas Represent Their Human Counterparts at the Molecular Level. |
|
