|
| Status |
Public on Apr 13, 2015 |
| Title |
NT_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Control Knock-down
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Primary Hepatocytes plated on collagen
|
| Treatment protocol |
treatment with 2'-O-(2-methoxy)ethyl phosphorothioate backbone oligonucleotides and RNAimax for 48 h
|
| Growth protocol |
Upon receival, media was changed to low glucose DMEM containing 10% serum, Penicillin/Streptomycin and antimycotics (GA-1000) for overnight acclimatization. Oligonucleotide treatments were initiated the following day for 48 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion WT kit
|
| Label |
Biotin
|
| Label protocol |
Labeling performed according to Affymetrix WT Plus reagent kit guidelines
|
| |
|
| Hybridization protocol |
Hybridization performed according to Affymetrix WT Plus reagent kit guidelines
|
| Scan protocol |
Scanning was performed on a GenChip Scanner 3000
|
| Description |
Gene expression data from primary hepatocytes from donor 3 treated with a non-target antisense nucleotide
|
| Data processing |
The data were analyzed using Affymetrix Expression Console using default settings
|
| |
|
| Submission date |
Sep 16, 2014 |
| Last update date |
Jan 31, 2024 |
| Contact name |
Ruth McPherson |
| E-mail(s) |
rmcpherson@ottawaheart.ca
|
| Organization name |
UOHI
|
| Street address |
40 Ruskin
|
| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1Y4W7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE61473 |
TRIB1 suppression on human hepatocytes transcriptome |
|
