|
| Status |
Public on Sep 20, 2014 |
| Title |
TE-8 [gene] |
| Sample type |
RNA |
| |
|
| Source name |
Esophageal squamous cell carcinoma-derived cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TE-8
cell type: esophageal squamous cell carcinoma-derived cell line
gender of donor: male
age of donor: 63
stage of escc: III
differentiation of escc: moderate
|
| Treatment protocol |
The cells underwent no treatment during culture.
|
| Growth protocol |
TE-1, TE-5, and TE-8 were cultured in RPMI-1640 supplemented with 10% fetal bovine serum. Het-1A was cultured using the BEGM kit (Lonza, Basel, Switzerland).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
AGPC method
|
| Label |
Cy3
|
| Label protocol |
Total RNAs (100 ng) were Cy3-labeled using the Low Input Quick Amp Labeling Kit, one-color (Agilent) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cRNA (600 ng) was applied for hybridization using the Gene Expression Hybridization Kit (Agilent) according to the manufacturer's instructions.
|
| Scan protocol |
Fluorescent signals were detected using a SureScan Microarray Scanner G4900DA (Agilent).
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to generate quantized raw data. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded. A global mean normalization method was used.
|
| |
|
| Submission date |
Sep 19, 2014 |
| Last update date |
Apr 23, 2018 |
| Contact name |
OSAMU ISHIBASHI |
| E-mail(s) |
ishibashi@biochem.osakafu-u.ac.jp
|
| Phone |
+81-72-254-9474
|
| Organization name |
Osaka Prefecture University
|
| Department |
Grad Sch Life & Environmental Sciences
|
| Lab |
Biological Macromolecules
|
| Street address |
1-1 Gakuen-cho, Naka-ku
|
| City |
Sakai |
| ZIP/Postal code |
599-8531 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16699 |
| Series (2) |
| GSE61587 |
Gene expression in normal and tumoral esophageal squamous cell lines |
| GSE61589 |
Gene and microRNA expression in normal and tumoral esophageal squamous cell lines |
|
| Relations |
| Reanalyzed by |
GSE113533 |
