|
| Status |
Public on Sep 20, 2014 |
| Title |
TE-8 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
Esophageal squamous cell carcinoma-derived cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TE-8
cell type: esophageal squamous cell carcinoma-derived cell line
gender of donor: male
age of donor: 63
stage of escc: III
differentiation of escc: moderate
|
| Treatment protocol |
The cells underwent no treatment during culture.
|
| Growth protocol |
TE-1, TE-5, and TE-8 were cultured in RPMI-1640 supplemented with 10% fetal bovine serum. Het-1A was cultured using the BEGM kit (Lonza, Basel, Switzerland).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
AGPC method
|
| Label |
Hy3
|
| Label protocol |
Total RNAs (250 ng) were 3’-labeled with Hy3 using the miRCURY LNA microRNA Hi-Power Labeling Kit (Exiqon) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The microarrays were hybridized with the denatured (95℃, 2 min) labeled RNAs at 56℃ for 16 hours with rotation.
|
| Scan protocol |
Fluorescent signals were detected using a SureScan Microarray Scanner G4900DA (Agilent).
|
| Data processing |
The raw data were re-scaled to account for the differences in individual hybridization intensities. Data were normalized based on a quantile normalization method.
|
| |
|
| Submission date |
Sep 19, 2014 |
| Last update date |
Sep 20, 2014 |
| Contact name |
OSAMU ISHIBASHI |
| E-mail(s) |
ishibashi@biochem.osakafu-u.ac.jp
|
| Phone |
+81-72-254-9474
|
| Organization name |
Osaka Prefecture University
|
| Department |
Grad Sch Life & Environmental Sciences
|
| Lab |
Biological Macromolecules
|
| Street address |
1-1 Gakuen-cho, Naka-ku
|
| City |
Sakai |
| ZIP/Postal code |
599-8531 |
| Country |
Japan |
| |
|
| Platform ID |
GPL19128 |
| Series (2) |
| GSE61588 |
MicroRNA expression in normal and tumoral esophageal squamous cell lines |
| GSE61589 |
Gene and microRNA expression in normal and tumoral esophageal squamous cell lines |
|
