|
| Status |
Public on May 06, 2015 |
| Title |
Clonal B-Cells from IgM Monoclonal Gammopathy of Undetermined Significance 33-99638_copy_number |
| Sample type |
genomic |
| |
|
| Source name |
Bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone marrow
cell type: Clonal B-Lymphocytes
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was digested with Nsp I and Sty I restriction enzymes, ligated to adapters with T4 DNA ligase and amplified. PCR products were concentrated and fragmented (DNAsal), and then end-labeled with deoxynucleotidil transferase
|
| Label |
biotin
|
| Label protocol |
Labelling and hybridizations were performed according to protocols from Affymetrix
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Data processing |
ChAS console from Affymetrix was used to assess genome wide copy number frequencies. Log2 Ratio copy number values with associated chromosome positions are available on the series record.
|
| |
|
| Submission date |
Sep 22, 2014 |
| Last update date |
May 06, 2015 |
| Contact name |
Bruno Paiva |
| E-mail(s) |
bpaiva@unav.es
|
| Organization name |
Clinica Universidad de Navarra
|
| Street address |
Av. Pio XII, 55
|
| City |
Pamplona |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL18637 |
| Series (2) |
| GSE61650 |
The cellular origin and malignant transformation of Waldenström's Macroglobulinemia [copy number variation] |
| GSE61651 |
The cellular origin and malignant transformation of Waldenström's Macroglobulinemia |
|
