|
| Status |
Public on Sep 25, 2014 |
| Title |
NOI003 |
| Sample type |
RNA |
| |
|
| Source name |
A549_uninfected
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
cell type: human lung epithelial cells
infected with: none (uninfected control)
|
| Treatment protocol |
Cells were grown in T-flasks (25 cm2) and identically processed by removing their growth medium, washed three times with PBS before adding serum-free medium containing hm/06 virus of 103 TCID50 for 36 h. The uninfected cells group as the mock simulated the procedure for virus infection.
|
| Growth protocol |
Human lung epithelial cells (A549) were cultured in F12 with 10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent (Invitrogen) and further purified with the RNeasy Mini Kit (Qiagen, Valencia, CA), following the manufacturer’s instructions.
|
| Label |
Hy3
|
| Label protocol |
The samples were labelled using the miRCURY™ Hy3™/Hy5™ Array Power Labelling Kit.
|
| |
|
| Hybridization protocol |
The samples were hybridized with the miRCURY™ LNA (locked nucleic acid) Array (v.11.0)
|
| Scan protocol |
Hybridizations between RNA samples and the spotted probes were performed with an Automated Hybridization Station (Lucidea Slide Processor; GE Healthcare, Piscataway, NJ). Signal scanning was performed with the Axon GenePix 4000B.
|
| Data processing |
Microarray Scanner and raw image intensities were read with GenePix Pro software, V6.0.
The signal intensity values obtained were normalized to per-chip median values and then applied to obtain geometric means and standard deviations for each miRNA
|
| |
|
| Submission date |
Sep 24, 2014 |
| Last update date |
Sep 25, 2014 |
| Contact name |
Zhao Lian Zhong |
| E-mail(s) |
626167675@qq.com
|
| Organization name |
Huazhong Agriculture University
|
| Street address |
Shizishan Street
|
| City |
Wuhan |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL19221 |
| Series (1) |
| GSE61701 |
microRNA (miRNA) expression profiling of A549 with H5N1 Influenza A Virus (hm/06)infection |
|
