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Sample GSM1511501 Query DataSets for GSM1511501
Status Public on Nov 22, 2016
Title Mist1-KrasG12D pancreatic fibroblasts, biological replicate 2
Sample type RNA
 
Source name Mist1-KrasG12D
Organism Mus musculus
Characteristics age: 23 weeks
tissue: Pancreas
genotype: Mist1-KrasG12D
Treatment protocol Fibroblasts were grown in 10% FBS DMEM in a 37° incubator with 7% CO2
Growth protocol Fibroblasts were cultured using a modified protocol for gravity separation.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix)
 
Hybridization protocol Following fragmentation, 15ug of cRNA was hybridized for 16 hours at 45°C on Affymetrix Mouse 430.2 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetric GeneChip Scanner 3000
Description Gene expression data from Mist1-KrasG12D pancreatic fibroblasts
Data processing Data were processed using Affymetrix Expression Console software. Robust Multichip Average (RMA) method was used to do background correction, quantile normalization, and expression level summarization.
 
Submission date Sep 24, 2014
Last update date Nov 22, 2016
Contact name Jason R Pitarresi
Organization name Ohio State University Medical Center
Street address 460 W. 12th Ave
City Columbus
State/province Ohio
ZIP/Postal code 43210
Country USA
 
Platform ID GPL1261
Series (1)
GSE61707 Smo and Ets-2 signaling in fibroblasts effects gene expression

Data table header descriptions
ID_REF
VALUE Quantile normalized probeset level expression values from Affymetrix Expression Console.

Data table
ID_REF VALUE
1415670_at 10.19161
1415671_at 11.06986
1415672_at 11.72539
1415673_at 9.722084
1415674_a_at 10.04618
1415675_at 9.221198
1415676_a_at 11.48611
1415677_at 9.526418
1415678_at 9.847645
1415679_at 10.93946
1415680_at 9.17819
1415681_at 9.487696
1415682_at 7.065833
1415683_at 11.27514
1415684_at 8.582554
1415685_at 8.22871
1415686_at 9.481078
1415687_a_at 12.6427
1415688_at 10.1488
1415689_s_at 8.639918

Total number of rows: 45101

Table truncated, full table size 894 Kbytes.




Supplementary file Size Download File type/resource
GSM1511501_I4__37.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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