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Status |
Public on Nov 22, 2016 |
Title |
Mist1-KrasG12D;FspCre;Ets2db/fl pancreatic fibroblasts, biological replicate 1 |
Sample type |
RNA |
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Source name |
Mist1-KrasG12D;FspCre;Ets2db/fl
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Organism |
Mus musculus |
Characteristics |
age: 23 weeks tissue: Pancreas genotype: Mist1-KrasG12D;FspCre;Ets2db/fl
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Treatment protocol |
Fibroblasts were grown in 10% FBS DMEM in a 37° incubator with 7% CO2
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Growth protocol |
Fibroblasts were cultured using a modified protocol for gravity separation.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix)
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Hybridization protocol |
Following fragmentation, 15ug of cRNA was hybridized for 16 hours at 45°C on Affymetrix Mouse 430.2 Array GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetric GeneChip Scanner 3000
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Description |
Gene expression data from Mist1-KrasG12D;FspCre;Ets2db/fl pancreatic fibroblasts
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Data processing |
Data were processed using Affymetrix Expression Console software. Robust Multichip Average (RMA) method was used to do background correction, quantile normalization, and expression level summarization.
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Submission date |
Sep 24, 2014 |
Last update date |
Nov 22, 2016 |
Contact name |
Jason R Pitarresi |
Organization name |
Ohio State University Medical Center
|
Street address |
460 W. 12th Ave
|
City |
Columbus |
State/province |
Ohio |
ZIP/Postal code |
43210 |
Country |
USA |
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Platform ID |
GPL1261 |
Series (1) |
GSE61707 |
Smo and Ets-2 signaling in fibroblasts effects gene expression |
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