|
| Status |
Public on Sep 26, 2014 |
| Title |
CD8+ T cells: SIV∆nef vaccinated macaque, 20 weeks post-vaccination biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
SIV∆nef vaccinated macaque blood CD8+ T cells
|
| Organism |
Macaca mulatta |
| Characteristics |
tissue: Blood
cell type: CD8+ T cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the Qiagen Rneasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard protocols described in the Affymetrix Gene Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rhesus macaque 3' IVT arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from SIV∆nef vaccinated macaque blood CD8+ T cells, 20 weeks post-vaccination
|
| Data processing |
The data were analyzed with BRB Array Tools, version 4.3
|
| |
|
| Submission date |
Sep 25, 2014 |
| Last update date |
Sep 26, 2014 |
| Contact name |
Michael George |
| E-mail(s) |
mdgeorge@ucdavis.edu
|
| Organization name |
University of California, Davis
|
| Department |
Medical Microbiology and Immunology
|
| Street address |
451 Health Sciences Drive
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE61766 |
Expression data from rhesus macaque blood CD8+ T cells |
|
