|
| Status |
Public on Sep 26, 2014 |
| Title |
neurons overexpressing EGFP rep3 |
| Sample type |
RNA |
| |
|
| Source name |
DRG neurons_overexpressing ECD
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain background: Sprague Dawley
development stage: embryo E16.5
genotype/variation: overexpressing ECD
tissue: dorsal root ganglis neuron
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed by agarose gel electrophoresis.
|
| Label |
streptavidin-Cy3
|
| Label protocol |
cRNA was biotin-UTP labelled using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin, TX).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
dissociated DRG neurons
|
| Data processing |
The raw data from the SampleProbeProfile txt file were exported from GenomeStudio software. Using the R beadarray package the data were log2 transformed, quantile normalized and filtered for detection p-value<0.01, therefore all probes with a detection p-value≥0.01 in all categories were excluded from the analysis.
|
| |
|
| Submission date |
Sep 25, 2014 |
| Last update date |
Sep 26, 2014 |
| Contact name |
Amelia Trimarco |
| E-mail(s) |
trimarco.amelia@hsr.it
|
| Organization name |
Ospedale San Raffaele
|
| Department |
Divisione di Neuroscienze-INSPE
|
| Lab |
unità interazioni axo-gliali
|
| Street address |
via olgettina 60
|
| City |
milano |
| ZIP/Postal code |
20132 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE61784 |
Gene expression profile in DRG neurons over-expressing NRG1 type III intracellular domain |
|
