tissue: fetal membranes neurocognitive impairment: CASE pair: 2
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from snap-frozen tissues using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and Qiagen RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturers’ recommendations.
Label
biotin
Label protocol
Total RNA (500 ng) was amplified and biotin-labeled with the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin, TX, USA).
Hybridization protocol
Standard Illumina hybridization protocol
Scan protocol
Standard Illumina scanning protocol
Description
CASE 9345725004_H
Data processing
Probes without an entry in the keys of the package illuminaHumanv4.db (Bioconductor) were removed. All values were offset by adding 1 and then log2 transformed. Quantile normalization was performed using normalize.quantiles function form the preprocessCore package.
