|
| Status |
Public on Sep 30, 2014 |
| Title |
Cancer tissue from patient 1046 |
| Sample type |
RNA |
| |
|
| Source name |
Cancer tissue from patient 1046
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
tumor stage: moderately differentiated
age: 45
afp: 1.5
distant metastasis: no
lymph node metastasis: no
vascular invasion: no
tumor size (centimeters): 6*5
prognosis: Survival
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the specimens using Trizol reagent (Invitrogen) and purified with mirVana miRNA Isolation Kit (Ambion, Austin, TX, USA). RNA purity and concentration were determined from OD260/280 readings using spectrophotometer (NanoDrop ND-2000) and RNA integrity was determined by 1% formaldehyde denaturing gel electrophoresis.
|
| Label |
Cy5
|
| Label protocol |
cDNA labeled with a fluorescent dye (Cy5 and Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction.
|
| |
|
| Hybridization protocol |
The labeled RNA was purified and hybridized to microarrays according to the Agilent manufacturer's instructions.
|
| Scan protocol |
Images were scanned with the Agilent G2565CA scanner (Agilent), gridded, and analyzed using Agilent feature extraction software version 10.7.
|
| Description |
raw file name: US10313827_253831410035_S01_GE2_107_Sep09_1_1
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Sep 29, 2014 |
| Last update date |
Sep 30, 2014 |
| Contact name |
wang jianguo |
| E-mail(s) |
21118059@zju.edu.cn
|
| Organization name |
zhejiang university
|
| Department |
Department of Surgery, First Affiliated Hospital, Zhejiang University School of Medicine
|
| Lab |
Key Lab of Combined Multi-Organ Transplantation
|
| Street address |
79 Qingchun Road
|
| City |
HANGZHOU |
| State/province |
ZHEJIANG |
| ZIP/Postal code |
310000 |
| Country |
China |
| |
|
| Platform ID |
GPL19243 |
| Series (1) |
| GSE61850 |
Integrated assessment of coding and non-coding expression in intrahepatic cholangiocarcinoma: Their potential biological significance as prognostic cancer biomarkers |
|
