|
| Status |
Public on Aug 01, 2008 |
| Title |
MMT + si Ctrl sample 3 |
| Sample type |
RNA |
| |
|
| Source name |
MMT
|
| Organism |
Mus musculus |
| Characteristics |
ATCC CCL-51
Organ: mammary gland
Disease: tumor
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
transfection by siRNA
|
| Growth protocol |
MMT were cultured on culture flasks (Becton Dickinson) in DMEM (Gibco, Invitrogen) supplemented with 10% (v/v) fetal calf serum (FCS, Gibco, Invitrogen), gentamycin (100 IU/ml) and non-essential amino acids (Gibco, Invitrogen)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from MMT cells using RNeasy kit (Qiagen) according to the manufacturer.
|
| Label |
digoxygenin
|
| Label protocol |
1µg were subjected to RT-IVT amplification and labeling according to the manufacturer (applied Biosystems, prodNo 4339628)
|
| |
|
| Hybridization protocol |
Labeled cRNAs were hybridized according to the supplied protocols (applied Biosystems, ProdNo 4346875)
|
| Scan protocol |
Applied Biosystems Expression Array System Software v1.1.1. (ProdNo: 4364137) has been used to acquire the chemiluminescence and fluorescence images and primary data analysis.
|
| Description |
Transcriptome profiles were acquired using novel Applied Biosystems AB1700 technology. All experimental data referred to as MGS V1.0 (Mouse Genome Survey Version 1, ProdNo: 4337467) were generated on the Applied Biosystems AB1700 transcriptome platform (ProdNo: 4338036). These arrays contain probes for 28,218 validated mouse genes.
|
| Data processing |
Applied Biosystems Expression Array System Software v1.1.1.
|
| |
|
| Submission date |
Dec 15, 2006 |
| Last update date |
Jul 29, 2008 |
| Contact name |
Anne Chotteau |
| E-mail(s) |
anne.chotteau@ibl.fr
|
| Phone |
0033320871128
|
| Fax |
0033320871111
|
| Organization name |
CNRS UMR 8161
|
| Lab |
Institut de Biologie de Lille
|
| Street address |
1 rue du professeur calmette
|
| City |
Lille |
| ZIP/Postal code |
59021 |
| Country |
France |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE6588 |
Target genes associated to siRNA mediated repression of Pea3 and Erm transcription factors in mammary cancerous cells |
|
