|
| Status |
Public on Oct 17, 2014 |
| Title |
RISC-free control transfected, biological rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
Human umbilical vein endothelial cell
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: RISC-free control transfected
cell type: Primary cell line
|
| Treatment protocol |
siRNA was synthesized as duplex by Life Technology and transfected directly using Invitrogen RNAiMAX. All steps were conducted according to the included vendor protocol.
|
| Growth protocol |
Cells were cultured in culture dishes pre-coated with 0.1% gelatin with commercial EGM2 media from Lonza
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen miRNeasy kit was used for extraction
|
| Label |
None
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol, performed by Penn Genomic Analysis Core
|
| Scan protocol |
standard Affymetrix protocol, performed by Penn Genomic Analysis Core
|
| Data processing |
standard Affymetrix protocol, conducted by Penn Bioinformatics Core and Dr. John Tobias
Expression level of genes in RISC-free or siYAP treated HUVEC
|
| |
|
| Submission date |
Oct 02, 2014 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Zhewei Shen |
| Organization name |
University of Pennsylvania
|
| Lab |
Ben Stanger, M.D., Ph.D.
|
| Street address |
BRB II/III Rm 527, 421 Curie Blvd.,
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE61989 |
Expression data from HUVEC with YAP siRNA knockdown |
|
| Relations |
| Reanalyzed by |
GSE86357 |
