|
| Status |
Public on Sep 25, 2020 |
| Title |
Retina from C3H WT mice, PN5, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Retinal RNA from C3H WT mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C3H
genotype/variation: WT
age: PN5
tissue: retina
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Neural retinas from wild-type control (C3Hwt/wt) and rd1 (C3Hrd1/rd1) mice were dissected at different post-natal days. Total RNA was purified onto cesium gradient.
|
| Label |
biotin
|
| Label protocol |
The cDNA was transcribed in vitro using RNA Transcript Labeling kit (ENZO Diagnostics, Farmingdale, NY) to form biotin-labeled cRNA
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on the GeneChip Array and the GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2007, Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip 3000 7G scanner
|
| Description |
Sample name: wtwt_d5_1
retina from WT C3H mouse aged 5 days
|
| Data processing |
Normalization: RMA, data analysis: R/Bioconductor
|
| |
|
| Submission date |
Oct 03, 2014 |
| Last update date |
Sep 25, 2020 |
| Contact name |
Frederic Blond |
| E-mail(s) |
frederic.blond@inserm.fr
|
| Organization name |
Institut de la Vision
|
| Street address |
17 rue Moreau
|
| City |
Paris |
| ZIP/Postal code |
75012 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE62020 |
Rod photoreceptors degeneration in the rd1 model of retinitis pigmentosa involves a cascade of events leading to proteasome dysfunction |
|
