|
| Status |
Public on Oct 08, 2015 |
| Title |
patient 219 with ALF - whole liver tissue mRNA - mRNA-ALF-219-2 |
| Sample type |
RNA |
| |
|
| Source name |
specimen obtained from explanted liver of patient 219
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole liver
phenotype: from OLT of HBV-associated acute liver failure
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 50 ng total RNA (GeneChip Expression Analysis Technical Manual, 2005-2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 to 18 µg (mean 12.8) of cRNA hybridized for 16 hr at 45°C on Affymetrics Human U133 Plus 2 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using GeneArray Scanner 3000_7Gplus
|
| Data processing |
Affymetrics probeset summaries were computed using RMA algorithm. Data were then imported to BRB-ArrayTools and subsequent data filtering, median normalization and statistical analyses were performed using this software.
|
| |
|
| Submission date |
Oct 03, 2014 |
| Last update date |
Oct 08, 2015 |
| Contact name |
Giacomo Diaz |
| E-mail(s) |
gdiaz@unica.it
|
| Organization name |
University of Cagliari
|
| Department |
Dept Biomedical Sciences
|
| Street address |
Via Porcell 4
|
| City |
Cagliari |
| ZIP/Postal code |
09124 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE62029 |
Integrated ordination of miRNA and mRNA expression profiles [mRNA] |
| GSE62037 |
Integrated ordination of miRNA and mRNA expression profiles |
|
