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Status |
Public on Oct 08, 2015 |
Title |
patient 29 liver donor - whole liver tissue mRNA - mRNA-DON-29 |
Sample type |
RNA |
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Source name |
specimen obtained from explanted liver of patient 29
|
Organism |
Homo sapiens |
Characteristics |
tissue: whole liver phenotype: liver donor
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 50 ng total RNA (GeneChip Expression Analysis Technical Manual, 2005-2006, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 10 to 18 µg (mean 12.8) of cRNA hybridized for 16 hr at 45°C on Affymetrics Human U133 Plus 2 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
Scan protocol |
GeneChips were scanned using GeneArray Scanner 3000_7Gplus
|
Data processing |
Affymetrics probeset summaries were computed using RMA algorithm. Data were then imported to BRB-ArrayTools and subsequent data filtering, median normalization and statistical analyses were performed using this software.
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|
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Submission date |
Oct 03, 2014 |
Last update date |
Oct 08, 2015 |
Contact name |
Giacomo Diaz |
E-mail(s) |
gdiaz@unica.it
|
Organization name |
University of Cagliari
|
Department |
Dept Biomedical Sciences
|
Street address |
Via Porcell 4
|
City |
Cagliari |
ZIP/Postal code |
09124 |
Country |
Italy |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE62029 |
Integrated ordination of miRNA and mRNA expression profiles [mRNA] |
GSE62037 |
Integrated ordination of miRNA and mRNA expression profiles |
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