|
| Status |
Public on Jan 18, 2007 |
| Title |
C2C12 Myotubes treated with Palmitate, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse C2C12 Myotubes treated with Palmitate for 16 hours
|
| Organism |
Mus musculus |
| Characteristics |
fully differentiated (day 6) myotubes
|
| Treatment protocol |
C2C12 myoblasts differentiated with 2% horse serum for 5 days. Treated for 16 hrs with 500 micromolar Palmitate. RNA collected using Rneasy (Qiagen) protocol.
|
| Growth protocol |
Grown in DMEM + 20% FBS to confluence. Then, media is changed to differentiation media (see above).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy kit protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse M430A version 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from Mouse C2C12 Myoblasts differentiated into Myotubes and treated for 16 hrs.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
|
| |
|
| Submission date |
Dec 20, 2006 |
| Last update date |
Mar 18, 2009 |
| Contact name |
Sarah Crunkhorn |
| E-mail(s) |
Sarah.Crunkhorn@joslin.harvard.edu
|
| Phone |
61726424795
|
| URL |
http://www.joslinresearch.org
|
| Organization name |
Joslin Diabetes Center
|
| Department |
CMP
|
| Lab |
Patti Lab
|
| Street address |
1 Joslin Place
|
| City |
boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE6766 |
C2C12 Myotubes in response to Palmitate |
|
