|
| Status |
Public on Jan 01, 2016 |
| Title |
control, 24h, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
whole cotton leaves_untreated control_24h
|
| Organism |
Gossypium hirsutum |
| Characteristics |
cultivar/variety: cv. CCRI12
tissue: whole cotton leaves
age: 6-7 fully expanded true leaf stage; 5-6 weeks after sowing
treated with: none (untreated control)
harvest time point: 24h after onset of herbivory
|
| Treatment protocol |
For insect treatment, seven Helicoverpa armigera larvae (third instars) were placed on a group of three plants, which were kept within plastic bags (30 × 40 cm), until time of harvest, with samples for each time point maintained separately. Undamaged plants maintained under the same conditions were used as controls. Cotton leaves from control plants and plants exposed to H. armigera were harvested at 6 h, 12 h, 24 h, and 48 h after onset of herbivory. For each treatment group and time point, cotton leaves were harvested from the three plants per treatment group and flash frozen in liquid nitrogen.
|
| Growth protocol |
Cotton seeds (Gossypium hirsutum cv. CCRI12) were grown in a growth chamber under 29/25°C temperature and a 16:8 h light:dark cycle, and water was added every two days. All plants were used in experiments at the 6-7 fully expanded true leaf stage, which occurred 5-6 weeks after sowing. Cotton bollworm (CBW; Helicoverpa armigera) larvae were reared on an artificial diet and maintained at 27 ± 2°C, 75 ± 10% relative humidity, and 14:10 h light:dark in the laboratory.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
A Modified hot borate method was used for RNA isolation.
|
| Label |
biotin
|
| Label protocol |
For each time point (6h, 12h, 24h, and 48h), the arrays were hybridized with Cy3 and Cy5 fluorescently labeled probe pairs of herbivore damaged plants plus the corresponding control. (Expression Analysis Technical Manual, 2001, Affymetrix). For each time point, three replicate treatments and controls were performed.
|
| |
|
| Hybridization protocol |
Cleaned total RNAs from each of the 24 samples were sent to CapitalBio Corporation in Beijing for microarray hybridization and preliminary data analysis.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Cotton plants under normal condition for 24h, biological replicate 3
Cotton plants (Gossypium hirsutum cv. CCRI12) were exposed to cotton bollworm (CBW; Helicoverpa armigera) larvae for 6h, 12h, 24h, and 48h (continuous feeding experiment). Control plants were harvested at the same time point. Whole cotton leaves were used for RNA isolation, labelling and array.
100807-10300F_C-24-3_(Cotton)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 08, 2014 |
| Last update date |
Jan 01, 2016 |
| Contact name |
xinzheng huang |
| E-mail(s) |
huangxinzheng85@163.com
|
| Organization name |
Institute of Plant Protection, Chinese Academy of Agricultural Sciences
|
| Street address |
No. 2 Yuanmingyuan West Road, Haidian District
|
| City |
beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL8672 |
| Series (1) |
| GSE62158 |
Dynamic transcriptome analysis and volatile profiling of Gossypium hirsutum in response to the cotton bollworm Helicoverpa armigera |
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