|
| Status |
Public on May 25, 2015 |
| Title |
Microglia_specificpathogenfree_6hr_after_LPS_ic_3 |
| Sample type |
RNA |
| |
|
| Source name |
Microglia, LPS i.c., SPF
|
| Organism |
Mus musculus |
| Characteristics |
cell type: microglia
treatment: LPS i.c.
condition: specifc pathogen free (SPF)
age: 6-8 weeks old
strain: C57BL/6
|
| Treatment protocol |
5 µg LPS were diluted in 10 µl PBS and applied intracranially under isofluoran anesthesia. Animals returned to their cages once they had recovered their righting reflex. 6 hours later, animals were analyzed. Control animals were injected with 10 µl sterile PBS i.c.
|
| Growth protocol |
Mice were housed under SPF or germ-free conditions. Animals were 6-8 weeks old.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Adult microglia were harvested using density gradient separation. CD11b+CD45lo microglial cell populations were FACS-sorted and collected directly in cell lysis buffer and subsequently RNA was isolated with the Arcturus Pico Pure RNA Isolation Kit (Life Technologies) according to the manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Total RNA was amplified using the Ovation PicoSL WTA System V2 and further processed using the Encore Biotin Module according to the instructions supplied by the manufacturer (NuGen).
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
|
| Scan protocol |
Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
|
| Description |
Microglia from specifc pathogen free (SPF) mice treated with LPS i.c.
|
| Data processing |
Background correction and normalization was performed using the RMA-Sketch implementation in Affymetrix Expression Console
|
| |
|
| Submission date |
Oct 09, 2014 |
| Last update date |
May 25, 2015 |
| Contact name |
Ori Staszewski |
| E-mail(s) |
ori.staszewski@uniklinik-freiburg.de
|
| Organization name |
University Medical Center Freiburg
|
| Department |
Institute of Neuropathology
|
| Street address |
Breisacher Str. 64
|
| City |
Freiburg |
| ZIP/Postal code |
D-79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17400 |
| Series (2) |
| GSE62201 |
Host microbiota constantly control maturation and function of microglia in the central nervous system (part 1) |
| GSE67858 |
Host microbiota constantly control maturation and function of microglia in the central nervous system |
|
