|
| Status |
Public on Oct 11, 2014 |
| Title |
TAp73+/+ hypoxia_8hr_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MEF_TAp73+/+ hypoxia_8hr
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse embryo fibroblast (MEF)
genotype/variation: TAp73+/+
culture condition: hypoxia (incubated in 1% O2)
time point: 8 hr
|
| Treatment protocol |
1% oxygen (hypoxia) for 4 or 8 hrs
|
| Growth protocol |
Cells were grown in 10% serum containing DMEM medium in 5%CO2 and 21% oxygen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All samples were snap frozen in liquid N2. Total RNA was extracted from all samples using Trizol and further purified by RNeasy RNA extraction kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
TA-WT-Hyp 8h 2.AVG_Signal
|
| Data processing |
The data were normalised using quantile normalisation with Partek Genomic Suites Version 6.6
|
| |
|
| Submission date |
Oct 10, 2014 |
| Last update date |
Oct 11, 2014 |
| Contact name |
Kanaga Sabapathy |
| Organization name |
National Cancer Centre Singapore
|
| Street address |
11 Hospital Drive
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
169610 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE62236 |
Expression data from TAp73+/+ and TAp73-/- MEFs |
|
