|
| Status |
Public on Sep 30, 2016 |
| Title |
Muscle at T3 post surgery, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Muscle at 3 days post overload
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Plantaris muscle
strain: Sprague Dawley
age: Retired breeders
|
| Treatment protocol |
Muscles were extracted at T0, T3, T7, or T28, and subsequently miced and homogenized in Qiazol. Total RNA was extracted using a miRNeasy mini kit (Qiagen)
|
| Growth protocol |
Achilles tendons were excised to ablate the gastrocnemius and soleus muscles, causing all plantar load to be placed on the plantaris muscle. This causes rapid hypertrophy of the plantaris muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiazol extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Affymetrix Plus WT kit protocol from 400 ng total RNA (GeneChip® WT Plus Reagent Kit Manual P/N 703174 Rev. 2).
|
| |
|
| Hybridization protocol |
Following fragmentation, 2.8 ug of cDNA were hybridized at 48C on Rat Gene 2.1 ST Array Plates using the Affymetrix GeneTitan system (software version 3.2.4.1515)
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneTitan system.
|
| Description |
Gene expression data from muscles at 3 days post overload
|
| Data processing |
Data was analyzed using the limma and oligo Bioconductor packages implemented in the R-statistical environment version 3.0.0. RMA was used to fit normalized log2 transformed expression values.
|
| |
|
| Submission date |
Oct 15, 2014 |
| Last update date |
Sep 30, 2016 |
| Contact name |
Jonathan Paul Gumucio |
| E-mail(s) |
jgumucio@umich.edu
|
| Phone |
734-764-4206
|
| Organization name |
University of Michigan
|
| Department |
Orthopaedic Surgery
|
| Street address |
109 Zina Pitcher Pl
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL19271 |
| Series (1) |
| GSE62388 |
Expression data from muscle samples following synergist ablation |
|
