|
| Status |
Public on Oct 23, 2014 |
| Title |
Test_sample_265 |
| Sample type |
RNA |
| |
|
| Source name |
Prostate cancer cells
|
| Organism |
Homo sapiens |
| Characteristics |
sample_id: 265
recurrence: yes
gleason grade: 7
t-stage: pT3a
margin status: Positive
preoperative psa: 10.7
|
| Treatment protocol |
Laser Micro dissection of prostate biopsies was performed using the PALM laser microbeam system
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy® Micro kit from Qiagen (Germany) folloed by mikroRNA extraction using the RNeasy micro kit .
|
| Label |
FAM
|
| Label protocol |
The miRNA was reverse transcribed using Megaplextm pool A and B (Applied Biosystems) followed by pre amplification. The cDNA pool was applied to the multiplex array cards and the experiment was run on the ABI 7900 HT platform using cycling conditions as provided by the manufacturer
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Test
|
| Data processing |
Normalization of results was done using RQ Manager (Applied Biosystems). A common threshold was established across all array cards for each miRNA, expression levels were normalized to MammU6 and average delta-Ct was used in downstream analyses
RQ Manager (Applied Biosystems) was used to compute normalized signals.
|
| |
|
| Submission date |
Oct 22, 2014 |
| Last update date |
Oct 23, 2014 |
| Contact name |
Lars Dyrskjot |
| E-mail(s) |
lars@clin.au.dk
|
| Organization name |
Aarhus University Hospital, Skejby
|
| Department |
Department of Molecular Medicine
|
| Street address |
Brendstrupgaardsvej
|
| City |
Aarhus N |
| ZIP/Postal code |
8200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL18942 |
| Series (1) |
| GSE62610 |
High miR-449b expression in prostate cancer is associated with biochemical recurrence after radical prostatectomy |
|
