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Sample GSM15338 Query DataSets for GSM15338
Status Public on Jan 10, 2005
Title MDS patient MDS1 (Repl. 2)
Sample type RNA
 
Channel 1
Source name Neutrophils from 7 healthy controls (RNA pooled)
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Neutrophils from MDS patient
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description The patient RNA was compared to the normal RNA pool using a T7-based amplification method: 1 microgram of total RNA was reverse-transcribed using a T7-oligodT primer and then labelled aRNAs produced via in vitro transcription in the presence of either Cy3-UTP or Cy5-UTP. The labelled aRNAs were then purified and hybridized competitively to the microarray.
 
Submission date Jan 16, 2004
Last update date May 28, 2005
Contact name Andrea Pellagatti
E-mail(s) andreapellagatti@yahoo.co.uk
Phone 00441865222911
Organization name University of Oxford
Department NDCLS, RDM
Lab LLR Molecular Haematology Unit
Street address John Radcliffe Hospital
City Oxford
ZIP/Postal code OX3 9DU
Country United Kingdom
 
Platform ID GPL334
Series (1)
GSE971 Myelodysplastic Syndromes (MDS) and Acute Myeloid Leukaemia (AML)

Data table header descriptions
ID_REF
CH1_NORM Channel 1 (Cy3, control) normalized intensity
CH2_NORM Channel 2 (Cy5, patient) normalized intensity
RATIO Ratio CH2/CH1
VALUE Log Ratio CH2/CH1

Data table
ID_REF CH1_NORM CH2_NORM RATIO VALUE
1 118.920166 112.6228535 0.947045882 -0.078493773
2 109.760506 110.0903106 1.003004765 0.00432846
3 197.100845 139.2989029 0.706739248 -0.500750065
4 128.273102 112.4211899 0.876420607 -0.190304689
5 142.974792 119.7561641 0.837603345 -0.255660891
6 133.878159 115.0991068 0.859730278 -0.218043979
7 157.659668 129.2344125 0.819704964 -0.28682336
8 161.579834 158.4195614 0.980441417 -0.028496665
9 135.575638 129.1922082 0.9529161 -0.069578898
10 163.58403 135.1436688 0.82614219 -0.275537985
11 299.495789 253.9665534 0.847980381 -0.237897208
12 1363.689087 835.3901226 0.612595738 -0.706992765
13 504.420166 519.5171087 1.0299293 0.042545306
14 155.294113 135.1952581 0.870575552 -0.199958589
15 120.58403 124.5914326 1.033233278 0.047166015
16 119.781509 127.9822134 1.06846386 0.095538111
17 131.558823 109.6822977 0.833712975 -0.262377307
18 355.016815 312.1961329 0.879384073 -0.185434692
19 120.365547 107.0840981 0.889657388 -0.168678242
20 289.731079 186.0148297 0.642025807 -0.639296806

Total number of rows: 9984

Table truncated, full table size 507 Kbytes.




Supplementary data files not provided
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