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Sample GSM15340 Query DataSets for GSM15340
Status Public on Jan 10, 2005
Title MDS patient MDS10 (Repl. 2)
Sample type RNA
 
Channel 1
Source name Neutrophils from 7 healthy controls (RNA pooled)
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Neutrophils from MDS patient
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description The patient RNA was compared to the normal RNA pool using a T7-based amplification method: 1 microgram of total RNA was reverse-transcribed using a T7-oligodT primer and then labelled aRNAs produced via in vitro transcription in the presence of either Cy3-UTP or Cy5-UTP. The labelled aRNAs were then purified and hybridized competitively to the microarray.
 
Submission date Jan 16, 2004
Last update date May 28, 2005
Contact name Andrea Pellagatti
E-mail(s) andreapellagatti@yahoo.co.uk
Phone 00441865222911
Organization name University of Oxford
Department NDCLS, RDM
Lab LLR Molecular Haematology Unit
Street address John Radcliffe Hospital
City Oxford
ZIP/Postal code OX3 9DU
Country United Kingdom
 
Platform ID GPL334
Series (1)
GSE971 Myelodysplastic Syndromes (MDS) and Acute Myeloid Leukaemia (AML)

Data table header descriptions
ID_REF
CH1_NORM Channel 1 (Cy3, control) normalized intensity
CH2_NORM Channel 2 (Cy5, patient) normalized intensity
RATIO Ratio CH2/CH1
VALUE Log Ratio CH2/CH1

Data table
ID_REF CH1_NORM CH2_NORM RATIO VALUE
1 160.424362 195.9927783 1.221714557 0.288907251
2 168.373947 206.0642416 1.223848732 0.291425252
3 349.147064 335.5461955 0.961045445 -0.057323441
4 193.159668 224.3102162 1.161268388 0.215701441
5 193 237.8529222 1.232398561 0.301468903
6 178.630249 208.8412241 1.169125752 0.225430116
7 201.033615 219.6362795 1.092535094 0.127679622
8 218.873947 245.5874264 1.122049608 0.166136462
9 186.235291 208.1372014 1.117603437 0.160408363
10 212.029419 249.6453501 1.177409019 0.235615584
11 407.920166 380.1442297 0.931908401 -0.101739938
12 1457.550415 986.5543239 0.67685777 -0.563075387
13 765.336121 559.6706225 0.731274282 -0.45151547
14 214.386551 222.4719446 1.03771409 0.053409008
15 161.605042 200.2755882 1.239290468 0.30951437
16 155.487396 210.5524053 1.354144521 0.437381719
17 175.41597 239.5738869 1.365747297 0.449690568
18 535.331909 468.2744922 0.874736746 -0.193079195
19 184.890762 245.4700846 1.327649267 0.408874072
20 471.928558 549.188484 1.163711063 0.218732897

Total number of rows: 9984

Table truncated, full table size 508 Kbytes.




Supplementary data files not provided
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