|
| Status |
Public on Oct 31, 2014 |
| Title |
MAP 3wpi-1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse spleen_MAP-infected_3wpi
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
gender: female
age: 5 weeks
infected with: Mycobacterium avium subsp. Paratuberculosis (MAP)
tissue: spleen
time point: 3 weeks post infection
|
| Treatment protocol |
Mice were intraperitoneally inoculated with 300 μL of PBS (control group) or a suspension of MAP ATCC 19698 (3 × 10^7 cells/mouse, MAP-challenged group). Five mice from each group were sampled at 3, 6, and 12weeks post-infection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the spleen of the experimental mice using RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturers’ protocol. After processing with DNase digestion, clean-up procedures, RNA samples were quantified, aliquot and stored at -80°C until use. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
MAP 3-1
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R 3.0.2
|
| |
|
| Submission date |
Oct 30, 2014 |
| Last update date |
Oct 31, 2014 |
| Contact name |
Min-Kyoung Shin |
| E-mail(s) |
mk6471@hanmail.net
|
| Organization name |
Seoul National University
|
| Street address |
1, Gwanak ro, Gwanak gu
|
| City |
Seoul |
| ZIP/Postal code |
151-742 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE62836 |
Global host transcriptional profiles following Mycobacterium avium subsp. paratuberculosis infection in mice |
|
