|
| Status |
Public on Jan 01, 2016 |
| Title |
Islet_Pax4/rtTA_-DOX_ Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Primary isolated islets from Pax4/rtTA mice untreated
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 2 months old
genotype/variation: Pax4/rtTA
treatment: untreated
tissue: Primary isolated islets
|
| Treatment protocol |
Animals were maintained under standard conditions
|
| Growth protocol |
Standard feeding
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from approximatelly 200 islets per replicate was isolated using the RNAesay micro kit according to the manufacturer´s protocol (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA Labeling Protocol (The Ambion WT Expression kit for Affymetrix GeneChip® Whole Transcript Expression Arrays, Life Technologies Corporation, 2009).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of single-stranded cDNA were hybridized for 16 hr at 45C on GeneChip® Mouse Gene 1,0 ST Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
|
| Scan protocol |
Microarrays were scanned using GeneChip® Scanner 3000 7G
|
| Description |
The mouse presented healthy appearance
|
| Data processing |
The .CEL files were generated using Affymetrix GCOS software and further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma package of R/Bioconductor project.
|
| |
|
| Submission date |
Oct 30, 2014 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Benoit Raymond Gauthier |
| E-mail(s) |
benoit.gauthier@cabimer.es
|
| Organization name |
Andalusian Center for Molecular Biology and Regenerative Medicine (CABIMER)
|
| Department |
Stem Cells
|
| Lab |
Pancreatic Islet Development and Regeneration Unit
|
| Street address |
Americo Vespucio Avenue
|
| City |
Seville |
| State/province |
Seville |
| ZIP/Postal code |
41092 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE62846 |
Regulation of ER Homeostasis and Cell Cycle by Pax4 Enhances β-Cell Survival and Protects Mice Against Experimental Autoimmune Diabetes |
|
