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Sample GSM1534790 Query DataSets for GSM1534790
Status Public on Oct 30, 2017
Title Heart_human_right atrial tissue_POAF_sample-5
Sample type RNA
 
Source name Pre-operative right atrial tissue from human diabetic patient in whom POAF developed
Organism Homo sapiens
Characteristics individual_time of day: Patient 11_AM
tissue: Heart_right atrial tissue
Growth protocol Prospective study. Right atrial appendage were processes immediately after collection in ice cold oxygenated buffer to extract RNA
Extracted molecule total RNA
Extraction protocol Extract-All extraction of total RNA was performed according to the manufacturer's instructions. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 50 ng RNA using the One-Color Low Input Quick Amp Labeling kit (Agilent) according to the manufacturer's instructions, followed by Absolutely RNA Nanoprep kit (Stratagene). Dye incorpor
 
Hybridization protocol 600ng of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 25 µl containing 25x Agilent fragmentation buffer and 10x Agilent blocking agent following the manufacturers instructions.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8x60k array slides, Scan resolution 3um, Dye channel is set to Green and Green PMT is set to 100%).
Description Right atrial tissue gene expression data from human diabetic patient in whom POAF developed
Data processing The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters (protocol GE1_107_sept09 and Grid: 03949_D_F_20120628) to obtain background subtracted and spatially detrended Processed Signal intensities. The processed data is available on the series level and contains the normalized signal intensity values.
 
Submission date Oct 30, 2014
Last update date Mar 06, 2018
Contact name Lefebvre Philippe
Organization name Inserm
Lab U1011
Street address Blvd Pr. J.Leclerc
City Lille
State/province Nord
ZIP/Postal code 59000
Country France
 
Platform ID GPL17077
Series (1)
GSE62871 Right atrial tissue gene expression data from human patients in sinus rhythm (SR) and patients whom post-operative atrial fibrillation (POAF) developed

Supplementary file Size Download File type/resource
GSM1534790_Heart_human_right_atrial_tissue_POAF-5.txt.gz 12.3 Mb (ftp)(http) TXT
Processed data are available on Series record

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