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Sample GSM15348 Query DataSets for GSM15348
Status Public on Jan 10, 2005
Title MDS patient MDS15 (Repl. 2)
Sample type RNA
 
Channel 1
Source name Neutrophils from 7 healthy controls (RNA pooled)
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Neutrophils from MDS patient
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description The patient RNA was compared to the normal RNA pool using a T7-based amplification method: 1 microgram of total RNA was reverse-transcribed using a T7-oligodT primer and then labelled aRNAs produced via in vitro transcription in the presence of either Cy3-UTP or Cy5-UTP. The labelled aRNAs were then purified and hybridized competitively to the microarray.
 
Submission date Jan 16, 2004
Last update date May 28, 2005
Contact name Andrea Pellagatti
E-mail(s) andreapellagatti@yahoo.co.uk
Phone 00441865222911
Organization name University of Oxford
Department NDCLS, RDM
Lab LLR Molecular Haematology Unit
Street address John Radcliffe Hospital
City Oxford
ZIP/Postal code OX3 9DU
Country United Kingdom
 
Platform ID GPL334
Series (1)
GSE971 Myelodysplastic Syndromes (MDS) and Acute Myeloid Leukaemia (AML)

Data table header descriptions
ID_REF
CH1_NORM Channel 1 (Cy3, control) normalized intensity
CH2_NORM Channel 2 (Cy5, patient) normalized intensity
RATIO Ratio CH2/CH1
VALUE Log Ratio CH2/CH1

Data table
ID_REF CH1_NORM CH2_NORM RATIO VALUE
1 328.281525 264.9385982 0.807046934 -0.309275519
2 324.705872 264.8317504 0.815605054 -0.29405738
3 672.962158 447.5228823 0.665004528 -0.588563931
4 382.886566 272.723053 0.712281592 -0.489480389
5 388.743683 286.5037522 0.73699912 -0.440265198
6 327.147064 257.8392431 0.788144756 -0.343467466
7 403.142853 314.0871999 0.779096535 -0.360125996
8 337.252106 275.994585 0.818362821 -0.289187491
9 328.319336 278.6093225 0.848592489 -0.236856186
10 360.06723 289.401326 0.803742473 -0.315194773
11 754.121826 492.7305348 0.653383204 -0.613998726
12 2927.705811 1755.260928 0.599534599 -0.738085081
13 1750.19751 1438.851368 0.822107996 -0.282600169
14 324.378143 272.4464935 0.839903981 -0.251703688
15 254.176468 244.300522 0.961145317 -0.057173524
16 251.605042 229.674417 0.9128371 -0.131570667
17 362.819336 339.9484293 0.936963374 -0.093935441
18 884.621826 464.4431343 0.525018851 -0.929558871
19 343.508392 284.0713251 0.826970554 -0.274092135
20 862.184875 1109.512632 1.286861628 0.363856934

Total number of rows: 9984

Table truncated, full table size 511 Kbytes.




Supplementary data files not provided
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