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Sample GSM15361 Query DataSets for GSM15361
Status Public on Jan 10, 2005
Title MDS patient MDS33 (Repl. 2)
Sample type RNA
 
Channel 1
Source name Neutrophils from 7 healthy controls (RNA pooled)
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Neutrophils from MDS patient
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description The patient RNA was compared to the normal RNA pool using a T7-based amplification method: 1 microgram of total RNA was reverse-transcribed using a T7-oligodT primer and then labelled aRNAs produced via in vitro transcription in the presence of either Cy3-UTP or Cy5-UTP. The labelled aRNAs were then purified and hybridized competitively to the microarray.
 
Submission date Jan 16, 2004
Last update date May 28, 2005
Contact name Andrea Pellagatti
E-mail(s) andreapellagatti@yahoo.co.uk
Phone 00441865222911
Organization name University of Oxford
Department NDCLS, RDM
Lab LLR Molecular Haematology Unit
Street address John Radcliffe Hospital
City Oxford
ZIP/Postal code OX3 9DU
Country United Kingdom
 
Platform ID GPL334
Series (1)
GSE971 Myelodysplastic Syndromes (MDS) and Acute Myeloid Leukaemia (AML)

Data table header descriptions
ID_REF
CH1_NORM Channel 1 (Cy3, control) normalized intensity
CH2_NORM Channel 2 (Cy5, patient) normalized intensity
RATIO Ratio CH2/CH1
VALUE Log Ratio CH2/CH1

Data table
ID_REF CH1_NORM CH2_NORM RATIO VALUE
1 245.247894 130.8304737 0.53346217 -0.906542128
2 252.357147 130.0781825 0.515452738 -0.956087942
3 675.642883 763.8267017 1.130518386 0.176984455
4 306.878143 193.6707248 0.631099768 -0.664060002
5 289.995789 200.5333626 0.691504395 -0.532189674
6 305.995789 172.6506174 0.564225468 -0.825656307
7 271.642853 159.385497 0.586746514 -0.76919073
8 280.226898 154.8197257 0.55247989 -0.856006143
9 298.155457 179.4892479 0.601998869 -0.732167318
10 326.264709 237.2115268 0.72705236 -0.459868829
11 880.920166 1263.79961 1.434635803 0.52068454
12 4341.062988 5771.46292 1.329504533 0.410888696
13 821.29834 1173.772869 1.429167468 0.515174979
14 359.491608 341.143608 0.948961256 -0.075578909
15 202.155457 135.4602595 0.670079658 -0.577595484
16 203.546219 142.1588453 0.698410641 -0.517852555
17 275.83194 163.0709219 0.591196661 -0.758289974
18 347.508392 291.4444314 0.838668758 -0.253826981
19 279.159668 159.3574793 0.570847073 -0.808823788
20 359.079834 295.7701062 0.823688991 -0.279828388

Total number of rows: 9984

Table truncated, full table size 508 Kbytes.




Supplementary data files not provided
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