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Sample GSM15363 Query DataSets for GSM15363
Status Public on Jan 10, 2005
Title MDS patient MDS4 (Repl. 2)
Sample type RNA
 
Channel 1
Source name Neutrophils from 7 healthy controls (RNA pooled)
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Neutrophils from MDS patient
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description The patient RNA was compared to the normal RNA pool using a T7-based amplification method: 1 microgram of total RNA was reverse-transcribed using a T7-oligodT primer and then labelled aRNAs produced via in vitro transcription in the presence of either Cy3-UTP or Cy5-UTP. The labelled aRNAs were then purified and hybridized competitively to the microarray.
 
Submission date Jan 16, 2004
Last update date May 28, 2005
Contact name Andrea Pellagatti
E-mail(s) andreapellagatti@yahoo.co.uk
Phone 00441865222911
Organization name University of Oxford
Department NDCLS, RDM
Lab LLR Molecular Haematology Unit
Street address John Radcliffe Hospital
City Oxford
ZIP/Postal code OX3 9DU
Country United Kingdom
 
Platform ID GPL334
Series (1)
GSE971 Myelodysplastic Syndromes (MDS) and Acute Myeloid Leukaemia (AML)

Data table header descriptions
ID_REF
CH1_NORM Channel 1 (Cy3, control) normalized intensity
CH2_NORM Channel 2 (Cy5, patient) normalized intensity
RATIO Ratio CH2/CH1
VALUE Log Ratio CH2/CH1

Data table
ID_REF CH1_NORM CH2_NORM RATIO VALUE
1 212.113449 226.309 1.066924333 0.093457863
2 244.542023 252.5691278 1.032825053 0.046595901
3 325.613434 395.1370976 1.213515956 0.279193078
4 237.172272 245.0777553 1.033332241 0.04730419
5 240.726898 224.0051283 0.930536347 -0.103865591
6 212.411758 211.5077411 0.995744035 -0.006153163
7 229.226898 240.1473706 1.04764045 0.067143669
8 243.726898 234.9800448 0.964112073 -0.052727233
9 230.613449 228.2146195 0.98959805 -0.015085437
10 265 286.0534418 1.07944695 0.110292343
11 338.247894 422.2441694 1.248327564 0.31999655
12 1130.735352 1713.174947 1.515098067 0.599411177
13 689.722717 1236.857751 1.793268107 0.842591198
14 237.252106 227.9171698 0.960653937 -0.057911282
15 226.970581 193.4447758 0.852290085 -0.230583546
16 206.588242 198.5667349 0.961171521 -0.057134192
17 227.457977 232.6156658 1.022675348 0.032348229
18 521.155457 599.8945359 1.151085588 0.202995108
19 255.743698 247.44717 0.967559208 -0.047578148
20 495.777313 378.5562516 0.763561062 -0.389184561

Total number of rows: 9984

Table truncated, full table size 507 Kbytes.




Supplementary data files not provided
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